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The collected ovaries were washed three times in sterile 0.9% sodium chloride and twice in phosphate buffered saline supplemented with 10% fetal bovine serum

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Though each compound acts through distinct biochemical pathways, their overlapping molecular targets reveal an integrative network capable of restoring insulin signaling, modulating steroidogenesis, and re-establishing ovulatory rhythm, reflecting a multidimensional redox-endocrine homeostasis that current pharmacotherapies only partially address

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